FFPE Sample Preparation and Pretreatment: For the RNAscope™ 2.5 Assay

This protocol is intended as a guide only, for full experimental details please read the product user manual. Chapter 1. Product InformationBefore using this product, read and understand the information inAppendix B. Safety on page20.IMPORTANT: We recommend reading the entire user manual before beginning any protocols.About this guideThis user manual provides guidelines and protocols for the proper preparation and pretreatment of formalin-fixed, paraffin-embedded (FFPE) tissues mounted on slides. The slides can then be assayed using an RNAscope™ 2.5 Reagent Kit.RNAscope™ 2.5 Reagent Kits come with a separate RNAscope™ 2.5 Assay User Manual. Do not perform an RNAscope™ 2.5 Assay without the correct user manual.Visit the document download section on the product page to download RNAscope™ 2.5 Assay User Manuals.Product descriptionBackgroundThe RNAscope™ 2.5 Assays use a novel and proprietary method of in situ hybridization (ISH) to visualize single RNA molecules per cell in samples mounte...