Oxidative Stress Assays and Oxidative Stress Markers

"We are currently using the product [ab113851] to measure microglial activation after 24 hours in response to activating stimuli. The product has been giving us very consistent results and is very easy to use.”- Dr Neal BennettDNA/RNA damage, lipid peroxidation, and protein oxidation/nitrationOxidative stress can be measured indirectly by measuring the levels of DNA/RNA damage, lipid peroxidation, and protein oxidation/nitration, rather than a direct measurement of reactive oxygen species. These oxidative stress markers are more enduring than reactive oxygen species.DNA/RNA damageThere are several types of DNA/RNA damage that can be measured as oxidative stress markers. 8-hydroxydeoxyguanosine (8-OHdG) is probably the most commonly used DNA damage marker for oxidative stress. Comet assays, assays for apurinic/apyrimidinic sites, and assays for aldehyde-induced damage can be used as less direct measures of DNA damage which is potentially related to oxidative stress.Lipid peroxidationMal...